Purification of beta-toxin from Clostridium perfringens type C.
نویسندگان
چکیده
Beta-toxin was purified about 340-fold from culture supernatant fluid of Clostridium perfringens type C with a yield of about 24% in terms of biologically active beta-toxin. The purification involved ammonium sulfate fractionation, gel filtration through Sephadex G-100, isoelectrofocusing in a pH 3 to 6 gradient, and immunoaffinity chromatography. The purified beta-toxin gave a single band on polyacrylamide gel electrophoresis.
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ورودعنوان ژورنال:
- Infection and immunity
دوره 18 3 شماره
صفحات -
تاریخ انتشار 1977